Functional characterization of the ER stress induced X-box-binding protein-1 (Xbp-1) in the porcine system
- Jin-Yu Zhang†1, 2,
- Kyu-Sun Lee†1,
- Ji-Su Kim3,
- Bong-Seok Song3,
- Dong-Il Jin2,
- Deog-Bon Koo4Email author and
- Kweon Yu1Email author
© Zhang et al; licensee BioMed Central Ltd. 2011
Received: 9 November 2010
Accepted: 24 May 2011
Published: 24 May 2011
The unfolded protein response (UPR) is an evolutionary conserved adaptive reaction for increasing cell survival under endoplasmic reticulum (ER) stress conditions. X-box-binding protein-1 (Xbp1) is a key transcription factor of UPR that activates genes involved in protein folding, secretion, and degradation to restore ER function. The UPR induced by ER stress was extensively studied in diseases linked to protein misfolding and aggregations. However, in the porcine system, genes in the UPR pathway were not investigated. In this study, we isolated and characterized the porcine Xbp1 (pXbp1) gene in ER stress using porcine embryonic fibroblast (PEF) cells and porcine organs. ER stress was induced by the treatment of tunicamycin and cell viability was investigated by the MTT assay. For cloning and analyzing the expression pattern of pXbp1, RT-PCR analysis and Western blot were used. Knock-down of pXbp1 was performed by the siRNA-mediated gene silencing.
We found that the pXbp1 mRNA was the subject of the IRE1α-mediated unconventional splicing by ER stress. Knock-down of pXbp1 enhanced ER stress-mediated cell death in PEF cells. In adult organs, pXbp1 mRNA and protein were expressed and the spliced forms were detected.
It was first found that the UPR mechanisms and the function of pXbp1 in the porcine system. These results indicate that pXbp1 plays an important role during the ER stress response like other animal systems and open a new opportunity for examining the UPR pathway in the porcine model system.
Endoplasmic reticulum (ER) is an essential cellular compartment for protein synthesis and maturation . The perturbation of ER functions such as disruption of Ca++ homeostasis, inhibition of protein glycosylation or disulfide bond formation, hypoxia, and bacteria infections results in the accumulation of unfolded or mis-folded proteins in ER lumen. To reduce the excessive mis-folded protein loading, cells trigger unfolded protein response (UPR) including the transient attenuation of protein translation, the degradation of mis-folded proteins, and the induction of molecular chaperones and protein folding enzymes . In severe ER stress, UPR results in cell death through the activation of apoptotic pathways .
Three distinct UPR signaling pathways exist in mammalian cells that include ER transmembrane inositol-requiring enzyme 1 (IRE1), PKR-like ER kinase (PERK), and activating transcription factor 6 (ATF6) pwthways . The evolutionary conserved IRE1 in the UPR pathway plays as a Ser/Thr protein kinase and endoribonuclease [4, 5]. Upon the activation of IRE1α by ER stress, the endonuclease domain of IRE1 splices the XBP1 mRNA and removes 26 base pairs from the full-length XBP1 mRNA by the unconventional splicing. This event results in the conversion of the premature unspliced XBP1 protein (XBP1u, 267 amino acids) to the spliced XBP1 protein (XBP1s, 371 amino acids) by the frame shift. XBP1s induces a subset of UPR target genes related to protein quality control, ER translocation, glycosylation, and ER/Golgi biogenesis [1, 6].
UPR has been intensively studied in various model systems including mice and human cell lines. However, the UPR pathway and its components have not yet been elucidated in the porcine model system. Pigs are an important resource in biomedical research because they are more similar to humans than the rodent model [7, 8]. Therefore, pigs have wide implications for studying human diseases such as diabetes obesity, hypertension, and cardiovascular diseases [9–11]. In addition, transgenic pigs are used as the bioreactor for the production of various growth hormones used in human medicine . Organs from cloned pigs produced by the somatic cell nuclear transfer (SCNT) can be used in xenotransplantation [13–15].
In this study, we report the function of pXbp1, the porcine ortholog of the human Xbp1, in ER stress. The unconventional splicing of pXbp1 mRNA is evolutionary conserved. In the RNA interference-mediated pXbp1 knock-down, we found that the pXbp1 participated in ER stress-mediated cell death through the regulation of the target gene expression. We also detected the spliced pXbp1S mRNA and protein in adult organs of pigs.
Tunicamycin induces UPR in PEF cells
To access whether ER stress response is activated in PEF cells by the TM treatment, we measured the expression levels of a ER stress marker BiP  and the activation of three major ER stress sensors IRE1α, PERK, and ATF6 with the Western blot analysis. BiP protein expression was significantly up-regulated from 3 h (Figure 1C). IRE1α and ATF6 were activated from 3 h and PERK was activated from 6 h after TM treatment (Figure 1D). These results indicate that ER stress induces unfolded protein response in PEF cells.
Cloning and sequence analysis of porcine Xbp1
Unconventional splicing of the porcine Xbp1
Nuclear localization of pXbp1 and ERAI vector system in PEF cells
Recently Iwawaki et al  reported the ER stress-activated indicator (ERAI) vector system for monitoring ER stress with the green fluorescence protein (GFP). In the ERAI plasmid, human Xbp-1-Venus fusion construct contains the partial sequence of the human XBP1 and a GFP variant (Venus) sequence. Upon ER stress, 26 nucleotides of human Xbp1 are spliced out and the XBP1-Venus fusion protein is produced. To test whether ERAI works as an indicator for ER stress in the porcine system, we checked the GFP signal in the ERAI transfected PEF cells after TM treatment. We found that 20.5% of total cells showed GFP signal in the TM treated ERAI-PEF cells, not in TM non-treated ERAI-PEF cells (Figure 4B, arrows). This observation was consistent with the results of the Western blot analysis. The band of the XBP-1-venus fusion protein (45 kDa) was very strong under ER stress (Figure 4C). These results suggest that the ERAI system can be used for monitoring the activation of UPR in the porcine system.
Porcine Xbp1 plays a role in ER stress-mediated cell death
Xbp1 activates its UPR target genes for maintaining the ER function and suppressing ER stress-induced cell death. The inhibition of Xbp1 activity by the dominant negative Xbp1 or knock-down Xbp1 markedly reduces the expression of Xbp1 dependent UPR target genes . Thus, we investigated the expression of pXbp1 target genes during ER stress response. The knock-down pXbp1 significantly reduced the expression of ERdj4 and p58IPK both in the normal condition and TM treated PEF cells (Figure 5B). In addition, well-known UPR target genes, including BiP, CHOP, and GRP94, were also significantly induced by TM treated PEF cells, but reduced in the pXbp1 knock-down PEF cells (Figure 5B). These results suggest that pXbp1 dependent target gene expression might be required for maintaining cell viability in PEF cells under the ER stress condition.
Porcine XBP1 as a UPR marker in the porcine system
The porcine model system is important to study human diseases because it shares similarities with humans in similar organ sizes, anatomy, and physiology . Comparative genomic analysis showed that porcine genomic sequences are more similar to those of the human than to the genomic sequences of the mouse . The UPR pathway is valuable for cellular homeostasis and animal development. Therefore, investigating the UPR pathway and monitoring ER stress in the model system is important to the study of pathogenic mechanisms that cause human diseases [1, 3, 26].
The results of this study show the UPR pathway in porcine system. As well as IRE1α-Xbp1 pathway, two ER transmembrane proteins, PERK and ATF6α, play important roles in the mammalian UPR. Upon UPR induction, ATF6α is released by BiP and exported to the Golgi, where it is cleaved by S1P proteases [5, 27]. Cleaved ATF6α acts as a transcriptional activator that can increase the expression of ER chaperones and Xbp1. In this study, we found that three UPR components (IRE1α, PERK, and ATF6α) are activated by ER stress in porcine cells (Figure 1D and 1E). Among these three UPR sensors, IRE1α-Xbp1 pathway plays a central role in ER stress response by the transcriptional regulation of genes involved in ER function. The amino acid sequence comparison and the phylogenetic analysis showed that pXbp1 has a high similarity to human Xbp1. In addition, the consensus sequence (CAGCAC/G) in Xbp1 mRNA of IRE1α target site is conserved between pigs and human (Figure 2). These results suggest that the IRE1α-Xbp1 pathway is evolutionary conserved in mammals.
Conventional splicing is catalyzed by the spliceosome for removing an intron within a primary transcript . In contrast, Xbp1 pre-mRNA is cleaved by IRE1α endonuclease-mediated unconventional splicing and this process is independent of the spliceosome [5, 29]. Unconventional splicing of Xbp1 mRNA leads to the production of an active transcription factor and induction of adaptive genes in the mammalian UPR. We showed that pXbp1 was also cleaved by unconventional splicing during ER stress response (Figure 3). Spliced pXbp1 mRNA was detected in the TM treated cells from 3 h and gradually increased until 9 h. In contrast, the protein level of active pXbp1s peaked within 3 h and slightly decreased from 6 h. During ER stress response, activated PERK pathway attenuates global protein translation by phosphorylating the eIF2α . We also detected that active PERK increased from 6 h in the TM treated cells (Figure 1D, E). Therefore, the translation of spliced and active Xbp1 mRNA may be attenuated by the PERK-eIF2α pathway.
In this study, we revealed that pXbp1 suppression is involved ER-mediated cell death through the regulation of target gene expression, such as ERdj4, p58IPK, and BiP. In addition, the ER stress-induced expression of CHOP, a pro-apoptotic transcription factor, was also reduced in the pXbp1 knockdown cell (Figure 5). The CHOP promoter contains ER stress response element (ERSE) and amino-acid-regulatory element (AARE). All three UPR transcription factors, ATF4, ATF6 and XBP1, can bind these promoter regions and regulate the transcription of CHOP . The expression of CHOP is reduced in Xbp1 knockout MEF cell line  and in pXbp1 knock-down porcine cells (Figure 5). These results indicate that pXbp-1 may be involved in the regulation of CHOP expression. However, PERK-ATF4 and ATF6α are known as dominant regulators for the CHOP expression rather than XBP1 [32, 33] and all three UPR pathways are required for the maximal induction of CHOP .
Studies in the mouse model show that the IRE1α-XBP1 pathway is essential for the liver and heart development and the function of secretion in pancreatic exocrine and plasma cells [22–24, 35]. The embryonic fibroblasts from XBP1-deficient mouse show defects in adipogenesis . We have investigated expression of the inactive and active isoforms of pXBP1 in various porcine tissues. While unspliced pXbp1 was expressed in all examined tissues, spliced pXbp1 was strongly expressed in liver and kidney (Figure 6A). Furthermore, active pXbp1s protein was detected in all examined tissues except heart (Figure 6B). This result indicates that pXbp1 plays a role in porcine tissues like other animals.
In this study, we show that unconventional splicing of pXbp1 mRNA is evolutionary conserved and pXbp1 is involved in ER-stress mediated apoptosis. In the adult porcine organs, spliced pXbp1 was expressed. These results suggest that pXbp1 can be used to monitor physiological and pathological ER stress in the porcine model system.
Cell culture and ER stress condition
Porcine embryo fibroblast (PEF) cells derived from 25-26 days of fetal pigs were cultured in Dulbecco's modified Eagle medium supplemented with 10% heat inactivated fetal bovine serum (FBS), 1% NEAA, 0.1% Gentamicine. PEF cells were maintained at 37°C in a humidified atmosphere of 5% CO2. In order to induce ER stress, PEF cells seeded in a 6-well dish were treated with Tunicamycin (TM, 2 μg/ml in DMSO) and harvested at 3 h, 6 h, and 9 h. Animal care and all experiments were conducted in accordance with KRIBB Guidelines for the Care and Use of Laboratory Animals and all experiments were approved by the institutional review board (KRIBB Institutional animal care and use committee/KRIBB-IACUC).
Cell viability assay
Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay using the CellTiter 96 Aqueous One kit (Sigma, St. Louis, MO, USA) according to the manufacturer's instructions.
Cloning of the porcine Xbp1 cDNA and phylogenetic analysis
To isolate porcine Xbp1, we searched the GenBank EST database with the BLAST (tblastn) program using the human XBP1 (GenBank accession number NM_005080) as the query sequence. LVRM100070G04, a Sus scrofa mRNA clone expressed in liver (GenBank accession number AK233029.1), showed high homology with the human XBP1 mRNA sequence. Oligonucleotide primers (pXBP1-F1, pXBP1-R1) were designed to clone the complete sequence of porcine Xbp1 mRNA (Figure 2A; Additional file 5, Table S1). PCR reaction was prepared with porcine cDNA generated from PEF cells, 1 pM of each primer, and 1X AccuPower® PCR premix (Bioneer, Daejeon, Korea). PCR Amplification was performed with 30 cycles of 94°C for 30 sec, 58°C for 30 sec, and 72°C for 2 min using GeneAmp PCR system 9700 (Applied Biosystems, Carlsbad, CA, USA). The cloned 1.7 kb PCR product was subcloned into the pGEM-T easy vector (Promega, Madison, WI) and was sequenced using an ABI automated sequencer (Applied Biosystem, Carlsbad, CA, USA). Homology searches for the cloned cDNA sequence and deduced amino acid sequences were performed by BLAST programs. Analysis of the primary structure of predicted proteins were performed by the SMART (Simple Modular Architecture Research Tool) at ExPASy Proteomics Server. Phylogenetic analysis was performed using ClustalW (http://www.ch.embnet.org). GenBank accession numbers for human XBP1: NM_002256, mouse Xpb-1: NM_178260, rat Xpb-1: NM_181692; bovine Xpb-1: XM_867473, Xenopus BK006297; zebrafish, EF690279; Drosophila, EF690279; C. elegansEF690279.
RNA preparation and reverse transcription-PCR analysis
The total RNA of PEF cells were extracted with Trizol reagent (Invitrogen, Carlsbad, CA, USA) and were further purified by On-Column RNase-free DNase digestion (Qiagen, Hilden, Germany) to remove possible genomic DNA contamination. cDNA was synthesized by using Superscript II® Reverse Transcription system (Invitrogen, Carlsbad, CA, USA). For RT-PCR, AccuPower® PCR premix (Bioneer, Daejeon Korea) was used with the set of primers. Primer sequences are in Additional file 5 (Table S1). Amplification conditions were as follows: a single cycle of 94°C for 5 min followed by 30 cycles of 94°C for 30s, 58°C for 30s, and 72°C for 30s, and the final single cycle of 72°C extension for 7 min. PCR products were separated by electrophoresis in the 2% agarose gel.
PEF cells were cultured on a gelatin-coated coverslip of up to 40% confluence. Cells were fixed in PBS contained 4% (w/v) formaldehyde for 30 min at room temperature (RT) and washed in PBS with 0.05% Tween 20 (PBST). After the incubation for 30 min in PBS containing 1% Triton X-100, the samples were blocked in PBST with 2% BSA for 1 h at RT, and incubated for 1.5 h at 37°C with a rabbit anti-Xbp1 antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Then, the cells were washed in PBST, incubated with a secondary antibody (1:200) for 1 h at 37°C, washed in PBST for 15 min four times, and mounted on Poly-prepslide glass (Sigma Aldrish, MO, USA) with a mounting media containing DAPI (Vectashield; Vector Laboratories, Burlingame, CA, USA). Samples were observed with a Karl Zeiss Axiovert 200 M fluorescence microscope equipped with an Apotome apparatus. Images were captured digitally using different filter sets and merged using AxioVision (v4.5) and Adobe Photoshop software (v7.0).
Western blot analysis
Protein preparation, SDS-PAGE, and immunoblotting were performed as previously described . KDEL (1:1000, Assay designs, Ann Arbor, MI, USA), phospho-PERK, PERK, IRE1α, ATF6α from (1:800, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-IIRE1α from (1:800, Abcam, Cambridge, MA, USA), ATF6α (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), XBP1 (1:1000, New England Biolabs, Ipswich, MA, USA) or β-actin (1:2000, Abcam, Cambridge, MA, USA) primary antibodies and horseradish peroxidase (HRP)-conjugated secondary antibodies (Santa Cruz Biotechology, Santa Cruz, CA, USA) were used.
Customized Stealth siRNA duplex oligonucleotides against pXbp1 (Genbank accession no. FJ213449.1) were synthesized by Invitrogen (Carlsbad, CA, USA). The sequences were as follows: sense 5'-UGAAGAGUCAACACCGUCAGA AUCC-3', antisense 5'-GGAUUCUGACGGU-GUUGACUCUUCA-3'. BLOCK-It Fluorescent Oligo (Invitrogen, Carlsbad, CA, USA) was used as the control. Before transfection, cells were plated at 50% confluency in Dulbecco's modified Eagle medium supplemented with 5% fetal bovine serum without antibiotics and incubated overnight. Transfections were performed with Lipofectamine™ RNAiMAX (Invitrogen, Carlsbad, CA, USA) as directed by the manufacturer. Transfection efficiencies were checked by the fluorescent-labeled control siRNA. The final concentration of the siRNA was 100nM. After 24 h incubation with siRNA, RT-PCR and cell viability assay were performed.
All experiments were repeated at least three times and the data was presented as the mean and error bar (± S.E.M.). The statistical significance was tested by Microsoft Excel-based application for the student t-test statistical analysis. A p-value of < 0.05 was considered to be significant.
Acknowledgements and Funding
We thank Dr. Masayuki Miura (University of Tokyo) for ERAI vector and Dr. O-Yu Kwon for comments on the manuscript. This work was supported by the grants from the National Research Foundation of Korea (2008-0061669, 2009-0080870 and 2010-0020964), the KRIBB Research Initiative Program, and BioGreen 21 Program (20070401034017) of the Republic of Korea.
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