Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]
© Singh et al; licensee BioMed Central Ltd. 2010
Received: 12 February 2010
Accepted: 21 November 2010
Published: 21 November 2010
Geranyl pyrophosphate (GPP) and p-hydroxybenzoate (PHB) are the basic precursors involved in shikonins biosynthesis. GPP is derived from mevalonate (MVA) and/or 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway(s), depending upon the metabolite and the plant system under consideration. PHB, however, is synthesized by only phenylpropanoid (PP) pathway. GPP and PHB are central moieties to yield shikonins through the synthesis of m-geranyl-p-hydroxybenzoate (GHB). Enzyme p-hydroxybenzoate-m-geranyltransferase (PGT) catalyses the coupling of GPP and PHB to yield GHB.
The present research was carried out in shikonins yielding plant arnebia [Arnebia euchroma (Royle) Johnston], wherein no molecular work has been reported so far. The objective of the work was to identify the preferred GPP synthesizing pathway for shikonins biosynthesis, and to determine the regulatory genes involved in the biosynthesis of GPP, PHB and GHB.
A cell suspension culture-based, low and high shikonins production systems were developed to facilitate pathway identification and finding the regulatory gene. Studies with mevinolin and fosmidomycin, inhibitors of MVA and MEP pathway, respectively suggested MVA as a preferred route of GPP supply for shikonins biosynthesis in arnebia. Accordingly, genes of MVA pathway (eight genes), PP pathway (three genes), and GHB biosynthesis were cloned. Expression studies showed down-regulation of all the genes in response to mevinolin treatment, whereas gene expression was not influenced by fosmidomycin. Expression of all the twelve genes vis-à-vis shikonins content in low and high shikonins production system, over a period of twelve days at frequent intervals, identified critical genes of shikonins biosynthesis in arnebia.
A positive correlation between shikonins content and expression of 3-hydroxy-3-methylglutaryl-CoA reductase (AeHMGR) and AePGT suggested critical role played by these genes in shikonins biosynthesis. Higher expression of genes of PP pathway was a general feature for higher shikonins biosynthesis.
Shikonins are red naphthoquinone pigments, which possess anti-microbial, anti-inflammatory and anti-tumour activities . These are active ingredient in several pharmaceutical and cosmetics preparations, and used as dye for fabrics and food items [1, 2]. Commercially, shikonins are extracted from roots of Lithospermum erythrorhizon with 0.14-1.09% yield . Arnebia [Arnebia euchroma (Royle) Johnston (family, Boraginaceae)] is another plant species that yields 1.58-1.94% shikonins from roots . The plant is naturally distributed in drier regions of Asia and Northern Africa . In India, it is well distributed in cold desert areas of Lahaul and Spiti district of Himachal Pradesh (latitude 32° 44' 57"-32° 59' 57" N; longitude 76° 46' 29" -78° 41' 34" E) at an altitude of >3800 m above mean sea level .
MEP pathway involves condensation of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate (DXP) using the enzyme DXP synthase (DXS). DXP yields 2-C-methyl-D-erythritol 4-phosphate (MEP) by a reaction catalysed by DXP reductoisomerase (DXR), and MEP is then transformed into IPP [8, 9] followed by its conversion into GPP, as in the MVA pathway.
Supply of GPP is critical in realizing the yield of isoprenoids , therefore, study on regulation of gene expression in GPP biosynthesis is of immense significance. Depending upon the metabolite and species under consideration, the preference for the route to GPP biosynthesis might differ. For example, natural rubber relies on MVA pathway, whereas stevioside is derived through MEP pathway [11, 12].
GPP and PHB are coupled through a reaction catalysed by p-hydroxybenzoate-m-geranyltransferase (PGT) to yield m-geranyl-p-hydroxybenzoate (GHB; Figure 1), which later leads to the biosynthesis of shikonins [13, 14]. Earlier work on shikonins biosynthesis was carried out in L. erythrorhizon using one gene of the MVA pathway (HMGR), three genes of PP pathway (PAL, C4H, and 4CL), and PGT. HMGR and PGT were shown to be regulatory genes in L. erythrorhizon[14, 15]. Information on other genes in relation to shikonins biosynthesis, however, has not been reported for L. erythrorhizon. Arnebia is an important source for shikonins with no molecular data on any of the genes involved in shikonins biosynthesis. Also, the relative importance of MVA or MEP pathway in relation to shikonins is not yet reported. Therefore, the present research was carried out in arnebia to (a) identify the major GPP synthesizing pathways (MVA versus MEP) contributing to shikonins biosynthesis, (b) clone various genes of the pathway, and (c) understand expression regulation of the genes of the identified pathway. The knowledge so generated will help to understand molecular basis of shikonins biosynthesis in arnebia and would lay basis of metabolic engineering for this important moiety.
Inhibitor studies suggested MVA as a preferred pathway for shikonins biosynthesis
To facilitate inhibitor studies, protocols for cell suspension culture were developed wherein shikonins content could be modulated. Shikonins content was recorded in traces (3.58 mgl-1) in suspension cultures maintained in growth medium [low shikonins production system (LSPS)]. The content, increased to 67.3 mgl-1 at day 1 of transfer of cultures from growth medium into production medium M9 [high shikonins production system (HSPS)]. Shikonins content increased to 478.8 mgl-1 at day 10 of the transfer to production medium M9 and declined thereafter, possibly due to senescence. The shikonins content remained in traces in LSPS during the entire period of experimentation.
At day zero (i.e. the day of transfer of culture from growth to production medium), when shikonins content was negligible, mevinolin (inhibitor of MVA pathway) and fosmidomycin (inhibitor of MEP pathway) were added separately at three different concentrations (50, 100 and 200 μM) and the samples were harvested at day 4 and day 8 of the treatment.
Twelve genes associated with MVA pathway, PP pathway and GHB biosynthesis were cloned
Since MVA was a predominant pathway for GPP supply for shikonins biosynthesis, all the genes of the pathway were cloned along with those associated with PP pathway and GHB biosynthesis. Degenerate primers for AeACTH, AeHMGS, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePAL, AeC4H and Ae4-CL yielded 540 bp, 413 bp, 474 bp, 286 bp, 495 bp, 620 bp, 361 bp, 809 bp, 270 bp and 531 bp sized amplicons, respectively (Additional file 1: Supplementary Table S1). Though in polymerase chain reaction (PCR) several amplicons were obtained in some of the cases, but the amplicons mentioned above were the ones which exhibited strong homology with the reported genes in the database. Partial gene sequences allowed designing of primers for rapid amplification of cDNA ends (RACE) to clone full-length genes. As mentioned in Methods section, availability of expressed sequence tag (EST) for AeHMGR and AePGT in the EST databank, facilitated designing of primers of these two genes for RACE.
Size and accession number of all the partial and full-length cDNAs are mentioned in Additional file 2: Supplementary Table S2. Ten full-length cDNAs namely, AeACTH (1.636 Kb), AeHMGR (2.007 Kb), AePMVK (1.745 Kb), AeMVDD (1.576 Kb), AeIPPI (894 bp), AeGDPS (1.483 Kb), AePGT (1.203 Kb), AePAL (2.380 Kb), AeC4H (1.767 Kb) and Ae4-CL (2.121 Kb) could be cloned. In spite of several attempts, full-length cDNAs of AeMVK and AeHMGS could not be cloned. Nucleotide and deduced amino acid sequences of the cloned genes are provided in Additional file 3: Supplementary Figure S1, whereas sequence alignment with the respective reported sequences from other plants is provided in Additional file 4: Supplementary Figure S2. Characteristics domains in deduced amino acid sequences of the genes are mentioned in Additional file 5: Supplementary Figure S3. SOPMA (Self-Optimized Prediction Method with Alignment) analysis for prediction of secondary structures in terms of helices, β turns, extended strands and random coils is provided in Additional file 6: Supplementary Figure S4).
Deduced amino acid sequence of all the genes possessed the required motifs and secondary structures (Additional file 5: Supplementary Figure S3 and Additional file 6: Supplementary Figure S4), which are essential to render characteristic functionality, as described previously in other plant systems. These examples include ACTH from Raphanus sativus; HMGS from Brassica juncea; HMGR; MVK, MVDD, IPPI, 4-CL, and PAL from Arabidopsis thaliana; GDPS from Abies grandis; C4H from Populus trichocarpa × Populus deltoides and PGT from L. erythrorhizon.
Down-regulation of all the twelve genes in response to mevinolin treatment
Correlation coefficient between gene expression and shikonins content in arnebia.
Name of the gene
Gene expression in cell culture treated with mevinolin
Gene expression in HSPS
Gene for coupling of MVA and PP pathways
Kinetics of gene expression vis-à-vis shikonins content in LSPS and HSPS identified critical genes
AePGT showed up-regulation by 141-184.5% in HSPS as compared to LSPS. The genes of PP pathway namely, AePAL, AeC4H and Ae4-CL also exhibited higher up-regulation of gene expression in HSPS as compared to LSPS at day 2 onwards. Expression of AeACTH, AeHMGS, AeMVK, AePMVK, AeMVDD, AeIPPI and AeGDPS was very similar in LSPS and HSPS during the entire period of experimentation (Figure 3a, b and Additional file 8: Supplementary Figure S6 showing IDV of Figure 3a, b). The correlation coefficient between shikonins content and IDV of the amplicons in HSPS had a value of 0.95, 0.91, 0.57, 0.59, and, 0.50 for AeHMGR, AePGT, AePAL, AeC4H and Ae4-CL, respectively (Table 1).
The PP and isoprenoid pathways synthesize array of secondary metabolites, which perform diverse functions in plants ranging from growth and development to defense. Some of these compounds have economic values and also have implications to human health. Red coloured, bioactive shikonins are such molecules which are used in food, fabric and pharmaceutical industries [1, 2].
Chemically, shikonins are composed of isoprenoid (e.g. GPP) and PHB moieties, which are also referred to as yield determining moieties . Plants have single PP pathway for the synthesis of PHB, whereas GPP is synthesised through MVA and MEP pathways. The preference for MVA versus MEP pathway depends upon the metabolites and the plant species under consideration [11, 12]. Coupling of the two moieties, GPP and PHB, is mediated through the enzyme PGT encoded by PGT.
Biosynthesis of shikonins predominantly uses MVA pathway for GPP supply
Relative contribution of MVA versus MEP pathway towards shikonins biosynthesis was assessed by using mevinolin and fosmidomycin, which are inhibitors of HMGR (of MVA pathway) and DXR (of MEP pathway), respectively [26, 27]. For such a study, a cell suspension culture-based system was developed wherein shikonins content increased from negligible quantity by 82.8 fold from day zero to day 10 of transferring the suspension cultures from LSPS to HSPS (Figure 3c). Inclusion of mevinolin produced a severe inhibition (92.82%) in shikonins accumulation (Figure 2a), whereas fosmidomycin produced a comparatively milder (49.97%) inhibition. Data thus implies a prominent role of MVA pathway in shikonins biosynthesis, though a role of MEP pathway can not be completely ruled out. The results are slightly different from L. erythrorhizon in which mevinolin inhibited the shikonins biosynthesis by 98%  suggesting that GPP for shikonins biosynthesis was derived from the MVA pathway. Involvement of both MVA and MEP pathway in terpenoid biosynthesis has been reported for dolichols biosynthesis in Coluria geoides, which might also be the case in arnebia.
Mevinolin down-regulated expression of all the twelve genes involved in GPP and GHB biosynthesis
Mevinolin is a known inhibitor of the enzyme HMGR. Down-regulation of expression of HMGR mRNA was reported in L. erythrorhizon in response to mevinolin treatment . Information on the effect of mevinolin on expression of other genes of shikonins biosynthesis pathway is not available in L. erythrorhizon. Our data showed that mevinolin down-regulated expression of all the eight genes involved in MVA pathway, all the three gene of PP pathway and AePGT (Figure 2b). Application of fosmidomycin had no effect on expression of these genes. Data suggested a substrate/product mediated feed-back and feed-forward regulation of the genes under study. For HMGR, its post-transcriptional and post-translational feedback regulation was suggested in the experiments using arabidopsis treated with lovastatin (analogous to mevinolin) . Previously, Dixon et al.  reported that endogenous cinnamate (product of PAL) caused inhibition of both transcription and enzymatic activity of PAL. Rani et al.  reported catechin-mediated down-regulation of several genes of PP pathway. In animal system, MVA pathway involved in the biosynthesis of cholesterol, has long been known to be regulated by end-product feedback inhibition and this regulation has been attributed to direct regulation of the expression of several cholesterol biosynthetic genes by the sterol sensing sterol regulatory element (SRE) binding protein-2 (SREBP-2) . In yeast and higher plants, MVK was subjected to feed-back inhibition by GPP and FPP [34, 35], suggesting feed-back inhibition by prenyl phosphates as a general regulatory mechanism to modulate the activity of MVK.
Existing literature thus supports the view of substrate mediated feedback inhibition, possibly that has been observed for HMGR, MVK and PAL. Overall down-regulation of other genes in response to mevinolin and fosmidomycin needs further investigations on substrate/product mediated feed-back and feed-forward regulation of the genes.
Not all genes exhibited up-regulation in HSPS
LSPS and HSPS provided a convenient system to study the possible regulatory genes in shikonins biosynthesis. Analysis of all the twelve genes showed that AeHMGR (of MVA pathway), and AePGT (involved in coupling of GPP and PHB) exhibited evident up-regulation in HSPS as compared to LSPS from day 2 onwards. Generally an up-regulation of all the genes of PP pathway was noticed in HSPS as compared to the LSPS. Earlier reports suggested HMGR enzyme to play a key role in the control of isoprenoid biosynthesis in plants [36, 37]. Lange et al.  proposed the importance of HMGR as an early enzyme in isoprenoid biosynthesis to control the metabolic flux into the MVA pathway, in contrast to the stringently regulated later enzymes controlling the biosynthesis of specific end products. Our data also suggested that AeHMGR could be an important regulatory gene in shikonins biosynthesis, since a strong positive correlation (r, 0.95) was obtained between its expression and the shikonins content (Table 1).
A requirement of higher PP pathway appeared to be general feature for shikonins biosynthesis in arnebia as evidenced by higher expression of the three genes (Figure 3). PP supplies PHB which is a substrate involved in shikonins biosynthesis [38, 39]. While in L. erythrorhizon genes of PP pathway did not exhibit any specific trend in relation to shikonins biosynthesis , data on arnebia appears to be in line with the previous reports on tea [32, 41] wherein all the three genes of PP pathway were reported to be regulatory.
AePGT encodes for PGT enzyme involved in GHB biosynthesis. This gene also showed up-regulation (three times) in HSPS and was positively correlated (r, 0.91) with shikonins content. Heide and Tabata  reported high (thirty five times) activity of PGT enzyme in shikonins producing culture extracts of L. erythrorhizon as compared to the non-shikonins producing system suggesting PGT to be important in regulating of biosynthesis of shikonins.
Arnebia prominently uses MVA pathway for the synthesis of GPP to be utilized in shikonins biosynthesis. Analyses of all the twelve genes suggested the importance of AeHMGR, all the genes of PP pathway and AePGT in realizing the shikonins yield in arnebia. This is the first report wherein twelve genes of shikonins biosynthesis pathway have been analysed to identify the regulatory genes and hence have implications in synthetic biology for shikonins production.
Plants of arnebia [Arnebia euchroma (Royle) Johnston] were procured from Kibber (a niche site of arnebia; latitude 32° 20' 11" N; longitude 78° 00' 52"E; 4200 m above mean sea level) in Lahaul and Spiti district of Himachal Pradesh, India and maintained in plastic pots (20 cm height x 20 cm top width x 12 cm bottom width) containing soil, sand, and farm yard manure mixture in a ratio of 2:1:1 at the Institute at Palampur (latitude 32° 06' 32" N; longitude 76° 33' 43" E; 1300 m above mean sea level).
Establishment of arnebia cell suspension cultures and inhibitor experiment
Composition of growth medium and production medium used for arnebia cell suspension culture.
Production medium (M9)
6-Benzyl amino-purine (BAP)
Indole-3-butyric acid (IBA)
In order to find out the possible role of MVA versus MEP pathway in shikonins production, different concentrations (50, 100 and 200 μM) of mevinolin (inhibitor of MVA pathway; dissolved in 100% ethanol and later added to the medium)  and fosmidomycin (inhibitor of MEP pathway; dissolved in water)  was added separately at the time of inoculation of cell biomass to M9 medium. Accordingly, two controls were used. One had equivalent concentration of ethanol as used for dissolving mevinolin (0.2%, 0.4% and 0.8% in respective control of 50, 100 and 200 μM mevinolin), and for other control equal volume of water, as used for dissolving fosmidomycin, was added to the medium. Cell suspension cultures were kept on shaker set at 100 rpm at 25°C under dark conditions. Samples were harvested at day 4 and day 8 of the treatment for shikonins analysis and gene expression studies.
Estimation of total shikonins
The production of shikonins in cell suspension cultures was measured as described by Yazaki et al. . For the purpose, 1 ml medium was aspirated from cell suspension culture and 500 μl of isoamyl alcohol was added followed by gentle shaking for 5 minutes, and allowed to settle till two layers were formed. A portion (200 μl) from the upper oily layer, was pipetted out to which 800 μl of freshly prepared KOH (2.5%) was added followed by gentle shaking for 5 minutes. After a while two layers got separated and the blue colored lower layer (500 μl) was used to record absorbance at 620 nm on a spectrophotometer (SPECORD 200, Analytik Jena, AG, Germany). A calibration curve was prepared using shikonin (Life Technologies, India) as standard.
Cloning of cDNAs involved in the biosynthesis of GPP, PHB and GHB
Degenerate primers were designed (Additional file 1: Supplementary Table S1) to clone AeACTH, AeHMGS, AeMVK, AePMVK, AeMVDD, AeGDPS, AeIPPI, AePAL, AeC4H, and Ae4-CL, based upon the conserved regions of corresponding genes reported for different plants. Total RNA was isolated from root tissue of arnebia using Colinmin™ and the iRIS™ system as described earlier [46–48]. RNA was treated with DNase I (amplification grade; Invitrogen, USA) and used for the synthesis of first strand complementary DNA (cDNA) using 0.5 μg oligo-d (T)12-18 primer (Invitrogen, USA) and 200 U Superscript II reverse transcriptase (Invitrogen, USA) in a total volume of 20 μl. Gene was amplified by PCR using 1 μl of cDNA template (prepared using RNA isolated from root tissue), 0.2 μM each of forward and reverse primers, 0.2 mM of dNTPs, 1 U of Taq DNA polymerase, and 1× PCR buffer in a final volume of 25 μl. Additional file 1: Supplementary Table S1 lists PCR conditions used to amplify the partial cDNAs. Amplified products were ligated into a pGEM®-T Easy vector (Promega, USA) and transformed into DH5α E. coli cells using the standard protocol. The transformed cells were spread on LB-ampicillin (100 μg/ml) plate along with isopropyl β-D-1-thiogalactopyranoside (IPTG) and 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal). All the white (transformed) colonies were re-streaked on a fresh ampicillin plate and colony PCR was performed to check the cloning of PCR product. PCR-positive colonies were used for isolation of plasmids using GenElute Plasmid Miniprep Kit (Sigma, USA). Sequencing of both the strands was performed using Big Dye terminator v3.1 cycle sequencing mix (Applied Biosystems, USA) on an automated DNA sequencer (ABI PRISM™ 310 and 3130 xl, Genetic Analyzer, Applied Biosystems, USA) and analyzed by BLAST algorithm at NCBI database.
Sequence of these partial cDNA was used for cloning of full length cDNAs using r apid a mplification of c DNA e nds (RACE). Primers for RACE of AeHMGR and AePGT were designed using their EST available at NCBI database vide accession number GR881971 and GR882046, respectively. Additional file 9: Supplementary Table S3 lists various primers and PCR conditions used for RACE. The 5'- and 3'- RACE-ready cDNAs were prepared and RACE was performed using SMART™ RACE cDNA Amplification Kit: Clontech, USA). Amplified products were cloned and analysed essentially as described above. Manufacturer's instructions were followed as and when necessary and also various protocols, as detailed in Sambrook et al. , were followed.
Primers were designed and analyzed using Primer 3 Input (Primer3_www.cgi v.0.2; http://frodo.wi.mit.edu/). Various sequences were aligned using ClustalW2 http://www.ebi.ac.uk/. Homology search was conducted using BLASTN and BLASTX algorithm http://www.ncbi.nlm.nih.gov/. Deduced amino acid sequence was used to analyse protein families and domains using tools available at PROSITE database at ExPASy Proteomics Server http://ca.expasy.org/, SMART http://smart.embl-heidelberg.de/ and NCBI conserved domain search http://www.ncbi.nlm.nih.gov/structure/cdd/. SOPMA http://npsa-pbil.ibcp.fr was used for secondary structure prediction of the deduced protein.
Semi-quantitative gene expression analysis
Complementary DNA was synthesized essentially as described earlier. PCR was carried out using gene specific primers and expression was evaluated at exponential phase of amplification as described earlier . Additional file 10: Supplementary Table S4 lists all the PCR parameters for reverse transcription-PCR (RT-PCR). Expression of 26S rRNA was used as internal control to equalize cDNA quantity in various reactions . Gel was viewed on a UV trans-illuminator and captured on gel documentation system (Alpha DigiDoc™, Alpha Innotech, USA). Integrated density value (IDV) of amplicons was calculated by AD-1000 software (Alpha DigiDoc™, Alpha Innotech, USA). The data was used to calculate the relative change in gene expression.
Gene expression in response to mevinolin and fosmidomycin
To study the gene expression in response to mevinolin and fosmidomycin (100 μM; the most effective concentration), RNA was isolated from arnebia cell suspension culture harvested at day 4 and day 8 day of the treatment, along with their respective controls and the expression was studied as described elsewhere.
Kinetics of gene expression and shikonins content in LSPS and HSPS
Cells were harvested at day 1, 2, 3, 4, 6, 8, 10 and 12 of transfer into production medium (HSPS). For control, the cells at similar day were harvested from growth medium (LSPS). RNA was isolated and expression analysis was performed by RT-PCR as described earlier. Shikonins content was also estimated on respective days as described elsewhere in the manuscript.
Authors are thankful to Council of Scientific and Industrial Research (CSIR) India, for funding the present research work under the mission mode project (CMM002) entitled "Developing Cell and Tissue Engineering for Colours and Dyes". RSS thanks University Grant Commission (UGC), India for award of junior/senior research fellowships. PKB and SM thank CSIR for awarding senior research fellowships. AK thanks CSIR for award of junior research fellowship. Technical help provided by Mr. Digvijay Singh and Ms. Anupama Saini in gene sequencing and plasmid isolation is duly acknowledged. The manuscript represents IHBT publication number 2022.
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